Basic Information

English Name: Pyroglutamic Acid p-Nitroanilide

CAS Number: 66642-35-1

Molecular Formula: C₁₁H₁₁N₃O₄

Molecular Weight: 249.2227

Appearance: White to light yellow solid

Purity: ≥99%

Applications

Biochemical Research: Pyroglutamic acid p-nitroanilide is commonly used in biochemical research as a chromogenic substrate for protein C and thrombin.

Urokinase Activity Assay: This compound can be used to detect urokinase activity. Urokinase is a serine protease that hydrolyzes pyroglutamic acid p-nitroanilide, releasing yellow p-nitroanilide. Urokinase activity can be quantified by colorimetric assays.

Other Applications: It can also be used to synthesize more complex peptide chain structures, for example as an intermediate in drug discovery.

Pyroglutamic acid p-nitroanilide is an important biochemical reagent widely used in urokinase activity assays and biochemical research. Its synthesis method is mature and exhibits high purity and stability.